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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Café. |
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Data corrente: |
22/06/2021 |
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Data da última atualização: |
23/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARÃES, P. S.; SCHENK, J. C. M.; CINTRA, L. C.; GIACHETTO, P. F.; SILVAROLLA, M. B.; PADILHA, L.; MALUF, M. P. |
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Afiliação: |
PAULA SOUZA GUIMARÃES, IAC; JULIANA CAMARGO MARTINATI SCHENK, IAC; LEANDRO CARRIJO CINTRA, CNPTIA; POLIANA FERNANDA GIACHETTO, CNPTIA; MARIA BERNADETE SILVAROLLA, IAC; LILIAN PADILHA, CNPCa; MIRIAN PEREZ MALUF, CNPCa. |
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Título: |
Large-scale prospection of genes on caffeine-free Coffea arabica plants - discovery of novel markers associated with development and secondary metabolism. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Plant Gene, v. 27, p. 1-9, Sept. 2021. |
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DOI: |
https://doi.org/10.1016/j.plgene.2021.100314 |
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Idioma: |
Inglês |
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Notas: |
Article 100314. |
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Conteúdo: |
Abstract. Differential gene expression profiles and metabolic networks are valuable tools for the genetic characterization of agronomic traits. In this study, we used large-scale expression analyses to identify modified biological processes in caffeine-free coffee plants. The first step was the large-scale sequencing of RNA from young and developing tissues of caffeine-free plants (AC1) and plants with normal concentrations of the compound (MN). The resulting 65,000 sequences were analyzed in silico for identification of 171 genes with differential expression between treatments, and establishment of metabolic networks associated with levels of caffeine. Few genes were mapped onto metabolic pathways, indicating that low caffeine has no major effects on physiological processes. The differential expression observed in silico was validated for 12 selected genes in field experiments using qPCR. The expression profile of 5 genes differed on the analyses, and the rest confirmed the in silico profile. Among the validated genes two of them, FIG and LSM-l, may control other agronomic traits associated with low caffeine content in coffee tissues. These genes are potential markers for use in association with other current markers for assisted selection of low-caffeine coffee. Therefore, they may improve the efficiency and effectiveness of coffee breeding programs. |
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Palavras-Chave: |
Assisted-selection; Caffeine-free plants; Coffee; Differential expression; Expressão gênica; RNAseq; Seleção assistida. |
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Thesagro: |
Café Descafeinado; Cafeína; Coffea Arábica. |
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Thesaurus Nal: |
Caffeine; Gene expression. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02480naa a2200361 a 4500
001 2132519
005 2021-06-23
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.plgene.2021.100314$2DOI
100 1 $aGUIMARÃES, P. S.
245 $aLarge-scale prospection of genes on caffeine-free Coffea arabica plants - discovery of novel markers associated with development and secondary metabolism.$h[electronic resource]
260 $c2021
500 $aArticle 100314.
520 $aAbstract. Differential gene expression profiles and metabolic networks are valuable tools for the genetic characterization of agronomic traits. In this study, we used large-scale expression analyses to identify modified biological processes in caffeine-free coffee plants. The first step was the large-scale sequencing of RNA from young and developing tissues of caffeine-free plants (AC1) and plants with normal concentrations of the compound (MN). The resulting 65,000 sequences were analyzed in silico for identification of 171 genes with differential expression between treatments, and establishment of metabolic networks associated with levels of caffeine. Few genes were mapped onto metabolic pathways, indicating that low caffeine has no major effects on physiological processes. The differential expression observed in silico was validated for 12 selected genes in field experiments using qPCR. The expression profile of 5 genes differed on the analyses, and the rest confirmed the in silico profile. Among the validated genes two of them, FIG and LSM-l, may control other agronomic traits associated with low caffeine content in coffee tissues. These genes are potential markers for use in association with other current markers for assisted selection of low-caffeine coffee. Therefore, they may improve the efficiency and effectiveness of coffee breeding programs.
650 $aCaffeine
650 $aGene expression
650 $aCafé Descafeinado
650 $aCafeína
650 $aCoffea Arábica
653 $aAssisted-selection
653 $aCaffeine-free plants
653 $aCoffee
653 $aDifferential expression
653 $aExpressão gênica
653 $aRNAseq
653 $aSeleção assistida
700 1 $aSCHENK, J. C. M.
700 1 $aCINTRA, L. C.
700 1 $aGIACHETTO, P. F.
700 1 $aSILVAROLLA, M. B.
700 1 $aPADILHA, L.
700 1 $aMALUF, M. P.
773 $tPlant Gene$gv. 27, p. 1-9, Sept. 2021.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
25/01/2018 |
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Data da última atualização: |
25/01/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TESK, C. R. M.; CAVALLI, J.; PEREIRA, D. H.; CARVALHO, P.; ALMEIDA, R. M. de; FARIA, A. C. de; RAMOS, T. A.; PEDREIRA, B. C. e. |
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Afiliação: |
CÁTIA R. M. TESK, UFMT-SINOP; JOSIANA CAVALLI, UFMT-SINOP; DALTON H. PEREIRA, UFMT-SINOP; PERIVALDO CARVALHO, UFMT-CUIABA; RONNY M. DE ALMEIDA, UFMT-SINOP; ARTUR C. DE FARIA, UFMT-SINOP; THAYS A. RAMOS, UFMT-SINOP; BRUNO CARNEIRO E PEDREIRA, CPAMT. |
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Título: |
Tussocks density, root and stubble mass of Quênia and Tamani guineagrass. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 54., 2017, Foz do Iguaçu. A new view of animal science: challenges and perspectives: Proceedings. Foz do Iguaçu: SBZ, 2017. p. 728. |
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ISSN: |
1983-4357 |
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Idioma: |
Inglês |
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Conteúdo: |
The forage use in animal production is functional and can be consider a strongly feed resource in the Brazilian livestock systems. Since grazing management has been studied, the understanding of organic reserves in forage plants is a challenge. The regrowth speed and longevity are function of recovery plant capacity after defoliation, what is highly influenced by harvest management. The objective with this study was to evaluate tussocks density, root and stubble mass Panicum maximum BRS Quenia and BRS Tamani. Pastures were submitted to intermittent grazing (95% of light interception) with two grazing intensities defined by postgrazing height: 20 and 35 cm for Quenia, and 15 and 25 cm for Tamani. The experiment was carried out at Embrapa Agrossilvipastoril, Sinop/MT, from March/15 to Dez/16, following a randomized complete block design, with tree replications. During the spring of 2016, after grazing, 3 tussocks were collect per experimental unit. Each tussock was fractionated in stubble (above soil) and root, washed and dried at 105 ° C for 1.5 hours and later at 55° for 72 hours. The tussocks quantification was done in 10 points per plot, using a frame of 1m². The data was analyzed using PROC MIXED of SAS statistical software. Treatments means were estimated by ?LSMEANS? compared by the probability of the difference (?pdiff?) at 5%. There is no cultivar and grazing intensity for root and stubble mass, and tussock density (P>0.05). Root mass was 1590 and 1915 kg ha-1 for Quenia and Tamani, respectively. The tussock density was, an average, 6.9 tussocks m-2, for both cultivars. Under lower intensity, the root and stubble mass were 1826 and 1293 kg ha-1, respectively. It did not differ in pastures under higher intensity, where 1683 and 1747 kg ha-1 were registered. Thus, Quenia and Tamani have well regrowth potential under different grazing intensity. The trigger for define the grazing end point for Tamani should be from 25 to 15 cm, and for Quenia from 35 to 20 cm,respectively. MenosThe forage use in animal production is functional and can be consider a strongly feed resource in the Brazilian livestock systems. Since grazing management has been studied, the understanding of organic reserves in forage plants is a challenge. The regrowth speed and longevity are function of recovery plant capacity after defoliation, what is highly influenced by harvest management. The objective with this study was to evaluate tussocks density, root and stubble mass Panicum maximum BRS Quenia and BRS Tamani. Pastures were submitted to intermittent grazing (95% of light interception) with two grazing intensities defined by postgrazing height: 20 and 35 cm for Quenia, and 15 and 25 cm for Tamani. The experiment was carried out at Embrapa Agrossilvipastoril, Sinop/MT, from March/15 to Dez/16, following a randomized complete block design, with tree replications. During the spring of 2016, after grazing, 3 tussocks were collect per experimental unit. Each tussock was fractionated in stubble (above soil) and root, washed and dried at 105 ° C for 1.5 hours and later at 55° for 72 hours. The tussocks quantification was done in 10 points per plot, using a frame of 1m². The data was analyzed using PROC MIXED of SAS statistical software. Treatments means were estimated by ?LSMEANS? compared by the probability of the difference (?pdiff?) at 5%. There is no cultivar and grazing intensity for root and stubble mass, and tussock density (P>0.05). Root mass was 1590 and 1915 kg ha-1 for Que... Mostrar Tudo |
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Palavras-Chave: |
Forage plants; Grazing intensities. |
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Thesaurus NAL: |
Forage; Grazing management. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171688/1/2017-cpamt-bruno-pedreira-tussocks-stubble-mass-quenia-tamani.pdf
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Marc: |
LEADER 02880nam a2200253 a 4500
001 2086383
005 2018-01-25
008 2017 bl uuuu u00u1 u #d
022 $a1983-4357
100 1 $aTESK, C. R. M.
245 $aTussocks density, root and stubble mass of Quênia and Tamani guineagrass.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 54., 2017, Foz do Iguaçu. A new view of animal science: challenges and perspectives: Proceedings. Foz do Iguaçu: SBZ, 2017. p. 728.$c2017
520 $aThe forage use in animal production is functional and can be consider a strongly feed resource in the Brazilian livestock systems. Since grazing management has been studied, the understanding of organic reserves in forage plants is a challenge. The regrowth speed and longevity are function of recovery plant capacity after defoliation, what is highly influenced by harvest management. The objective with this study was to evaluate tussocks density, root and stubble mass Panicum maximum BRS Quenia and BRS Tamani. Pastures were submitted to intermittent grazing (95% of light interception) with two grazing intensities defined by postgrazing height: 20 and 35 cm for Quenia, and 15 and 25 cm for Tamani. The experiment was carried out at Embrapa Agrossilvipastoril, Sinop/MT, from March/15 to Dez/16, following a randomized complete block design, with tree replications. During the spring of 2016, after grazing, 3 tussocks were collect per experimental unit. Each tussock was fractionated in stubble (above soil) and root, washed and dried at 105 ° C for 1.5 hours and later at 55° for 72 hours. The tussocks quantification was done in 10 points per plot, using a frame of 1m². The data was analyzed using PROC MIXED of SAS statistical software. Treatments means were estimated by ?LSMEANS? compared by the probability of the difference (?pdiff?) at 5%. There is no cultivar and grazing intensity for root and stubble mass, and tussock density (P>0.05). Root mass was 1590 and 1915 kg ha-1 for Quenia and Tamani, respectively. The tussock density was, an average, 6.9 tussocks m-2, for both cultivars. Under lower intensity, the root and stubble mass were 1826 and 1293 kg ha-1, respectively. It did not differ in pastures under higher intensity, where 1683 and 1747 kg ha-1 were registered. Thus, Quenia and Tamani have well regrowth potential under different grazing intensity. The trigger for define the grazing end point for Tamani should be from 25 to 15 cm, and for Quenia from 35 to 20 cm,respectively.
650 $aForage
650 $aGrazing management
653 $aForage plants
653 $aGrazing intensities
700 1 $aCAVALLI, J.
700 1 $aPEREIRA, D. H.
700 1 $aCARVALHO, P.
700 1 $aALMEIDA, R. M. de
700 1 $aFARIA, A. C. de
700 1 $aRAMOS, T. A.
700 1 $aPEDREIRA, B. C. e
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Embrapa Agrossilvipastoril (CPAMT) |
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